min mAU mAU min min mAU um 5 um Acetamidophenol 2 Caffeine 3 2-Acetamidophenol 4 Acetanilide 5 Acetylsalicylic Acid 6 Phenacetin 7 Salicylic Acid LC Conditions Column: SB-C18, 4.6 x 30 mm Detector: 254 nm Injector: 1 ul, Mobile Phase: 1% Formic Acid Acetonitrile (82:18) Flow: 2.0 ml/min 1.8 um Aumento della Risoluzione in funzione del Particle Size

Meno particelle più piccole Piccola quantità di particelle più grandi

min µm:Rs(4,5) = 5.45 Peak Width 5s(9) = µm: Rs(4,5) = 3.63 Peak Width, 5s (9) = Rs = +50% Aumento della Risoluzione in funzione del Particle Size + 50% Resolution (Theory: + 67%) + 125% Efficiency (Theory: + 177%) Colonna SB-C18 RRHT 4.6 x150mm Massima lunghezza

Rs(1,2) = x 250 mm, 5  m 1 mL/min min x 100 mm, 3.5  m 1 mL/min 4.6 x 30 mm, 1.8  m 1 mL/min 4.6 x 30 mm, 1.8  m 2 mL/min min min min Rs(1,2) = 4.8 Rs(1,2) = 3.5 Rs(1,2) = 3.3 N=22680 N=11691 N=6104 N=6568 N=6460 N=6463 N= HT + 1.8um RRHT : Reduction of analysis time from 30 min to 2 min Speed: 14x faster than 4.6 x 250mm, 5um Time saving: 92% Resolution: -35% AUMENTO DELLA VELOCITA’ D’ANALISI CON HPLC 1100

Speed Sample: 7 Analgesics Column: Zorbax RRHT SB-C18, 1.8 um, 4.6 x 30 mm Rs 2,1 = 2.1 Rs 2,1 = 2.2 Rs 7,6 = 2.2 Higher flow rate: higher speed – same resolution – higher sensitivity/precision 2 ml/min – 90bar 3 ml/min – 133bar 4 ml/min – 181bar 2x faster than 2ml/min without loss in resolution! Ultra-fast analysis in 26 sec 4  peak width ~ 0.6 sec < 50 sec cycle time 7 analgesics separated in 21 sec for peaks 1,2,3 Total time Injection-to-Injection

HPLC, 40°C SB-C x 150mm, 5µm 1.20ml/min, 40°C Analysis Time = 11min min Rapid Resolution HT Columns – Up to 20X Faster RRHT SB-C18 2.1mm x 50mm 1.8µm 1.00ml/min, 40°C Analysis Time= 1.1min RRLC, 40°C 10x faster min min RRHT SB-C18 2.1mm x 50mm 1.8µm 2.40ml/min, 95°C Analysis Time: 0.4min RRLC, 95°C 27x faster >20X faster Up to 20x faster than conventional HPLC With same or better performance (resolution, precision, sensitivity, carry over) Compliant with strictest regulatory performance requirements

min µm:Rs(4,5) = 5.45 Peak Width 5s(9) = µm: Rs(4,5) = 3.63 Peak Width, 5s (9) = Rs = +50% NON SOLO VELOCITA’ MA ANCHE EFFICIENZA + 50% Resolution (Theory: + 67%) + 125% Efficiency (Theory: + 177%) Colonna SB-C18 RRHT 4.6 x150mm Massima lunghezza