INFLUENCE OF MCM-41 PARTICLES MORPHOLOGY AND FUNCTIONALIZATION ON IBUPROFEN UPTAKE AND RELEASE VI Convegno Nazionale sulla Scienza e Tecnologia dei Materiali. Perugia giugno 2007 Valentina Aina 1.V. Aina, G. Cerrato, G. Magnacca, C. Morterra Dip. Chimica IFM, Università di Torino, Torino-Italy 2.V. Cauda, B. Onida, E. Garrone Dip. di Scienza dei Materiali e Ingegneria Chimica Politecnico di Torino, Torino-Italy 3.F. Balas, M. Colilla, M. Manzano, M. Vallet-Regì Dpto. de Quimica Inorganica y Bioinorganica Universidad Complutense de Madrid, Madrid-Spain 4.M. Rodriguez, C. Otero Dpto de Quimica, Universidad de las Islas Baleares, Palma de Mallorca-Spain

VI Convegno Nazionale sulla Scienza e Tecnologia dei Materiali. Perugia giugno 2007 Particles morphology influence for drug release Irregular particles Spherical particles Functionalization influence for drug release Si OH Si Ordered mesoporous silica: MCM-41 Ordered mesoporous silica allow to adsorb and deliver a drug in a homogeneous and controlled way

VI Convegno Nazionale sulla Scienza e Tecnologia dei Materiali. Perugia giugno 2007 Sphericals particles 490 nm 615 nm 770 nm Name samplesDescription MCM-41 irrMCM-41 particles of irregular shape MCM-41 sph. 490 nmMCM-41 particles of spherical shape, with dimension of 490 nm MCM-41 sph. 615 nmMCM-41 particles of spherical shape, with dimension of 615 nm MCM-41 sph. 770 nmMCM-41 particles of spherical shape, with dimension of 770 nm Irregulares particles SEM

VI Convegno Nazionale sulla Scienza e Tecnologia dei Materiali. Perugia giugno 2007 Samples Dp (nm) Vp (cm 3 /g) S BET (m 2 /g) MCM-41 sph 490nm2,10,46874 MCM-41 sph 615nm1,780, MCM-41 sph 770nm1,950, Samples Dp (nm) Vp (cm 3 /g) S BET (m 2 /g) MCM-41 IRR3,40,80928 Samples characterization

VI Convegno Nazionale sulla Scienza e Tecnologia dei Materiali. Perugia giugno 2007 [drug] mesoporous  [drug] solvent Uptake conditions: mg ibuprofen/ mg material = 1 Solution of ibuprofen with n-pentane (33 mg/ml) 24h with stirring (two methods): - magnetic stirring - orbital stirring mesoporous material drug solution Samples % ibuprofen (TGA/DTA) MCM-41 Irr+ Ibu21% MCM-41 sph 490 nm + Ibu11% MCM-41 sph 615 nm + Ibu15% MCM-41 sph 770 nm + Ibu17% Samples % ibuprofen (TGA/DTA) MCM-41 Irr+ Ibu26% MCM-41 sph 490 nm + Ibu26% MCM-41 sph 615 nm + Ibu25% MCM-41 sph 770 nm + Ibu25% Magnetic stirring Orbital stirring Ibuprofen Uptake

VI Convegno Nazionale sulla Scienza e Tecnologia dei Materiali. Perugia giugno 2007  2.5 nm Samples Dp (nm) Vp (cm 3 /g) S BET (m 2 /g) MCM-41 IRR3,40,80928 MCM-41 IRR + Ibu2,50,32514 MCM-41 IRR after release ibu/0,33134

VI Convegno Nazionale sulla Scienza e Tecnologia dei Materiali. Perugia giugno 2007 mesoporous material + drug Release conditions Samples Simulated biological fluids (SBF) at 37ºC and pH 7,4 Continuos stirring 50 mg of samples with 14 ml of SBF Ibuprofen released monitored with UV-Vis Ibuprofen release SBF solution

VI Convegno Nazionale sulla Scienza e Tecnologia dei Materiali. Perugia giugno 2007 Ibuprofen release Release fast 80% of ibuprofen released during the first 9 hours 15% of ibuprofen released more slowly Spherical particles release the drug more slowly then irregular particles Smaller are the spherical particles, slower is the ibuprofen release

VI Convegno Nazionale sulla Scienza e Tecnologia dei Materiali. Perugia giugno 2007 R The aim is to integrate organic groups onto the pores surface through Si-C covalent bonds, oriented towards the inner axes of the channels. Functionalized mesoporous materials High degree of functionalization Homogeneous distribution throughout the pores surface To preserve the mesoporous structural arrangement due to its significance in future applications Requisites

VI Convegno Nazionale sulla Scienza e Tecnologia dei Materiali. Perugia giugno 2007 Functionalization vs ibuprofen interaction MCM-41 functionalization with AP-TES: to investigate the effects towards ibuprofen storage and release. Amount of AP-TES Samples % func. (TGA/DTA) MCM-41 Irregular Fun.13% MCM-41 spher 490 nm + Fun12% MCM-41 spher 615 nm + Fun13% MCM-41 spher 770 nm + Fun13% Samples%C%H%N MCM-41 Irregular Fun9,912,753,26 MCM-41 spher 490 nm + Fun8,462,382,76 MCM-41 spher 615 nm + Fun9,522,743,04 MCM-41 spher 770 nm+ Fun9,282,612,97

VI Convegno Nazionale sulla Scienza e Tecnologia dei Materiali. Perugia giugno 2007 Samples Dp (nm) Vp (cm 3 /g) S BET (m 2 /g) MCM-41 IRR3,40,80928 MCM-41 IRR + fun.2,690,23341 MCM-41 IRR + fun + ibu-0,0530 MCM-41 IRR + fun after ibu rel-0,24122 Samples characterization

VI Convegno Nazionale sulla Scienza e Tecnologia dei Materiali. Perugia giugno 2007 After the functionalization the release profile is slower for all the samples After 9 hours the 50% of drug was released It can be clearly seen that spherical particles release more slowly than irregular particles Ibuprofen release

VI Convegno Nazionale sulla Scienza e Tecnologia dei Materiali. Perugia giugno 2007 IBUPROFEN UPTAKE The ibuprofen uptake is the same in all samples with magnetic stirring. It can be seen the influence of particles morphology in the uptake with orbital stirring. The amount of ibuprofen uptake is slower with orbital stirring than magnetic stirring, in the same conditions. Conclusions Spherical particles release the drug more slowly then irregular particles. Smaller are the spherical particles, slower is the ibuprofen release. After the functionalization the release profile is slower for all the samples. IBUPROFEN RELEASE

VI Convegno Nazionale sulla Scienza e Tecnologia dei Materiali. Perugia giugno 2007 thank you for your attention! Research funded by MIUR (Project COFIN2006, Prot , Area 03 ), Consortium INSTM and Center of Excellence for Nanostructured Interfaces and Surfaces (University of Torino, Italy). Acknowledgements