L’ ‘epigenetica’ si riferisce a tutti i cambiamenti dell’espressione genica e dell’organizzazione della cromatina che sono indipendenti dalla sequenza del DNA.
Epigenetics -DNA methylation (5-mC, 5-hmC) -Histone PTMs (>100)
3 Years old 50 Years old
Age Dependent Epigenetic differences in Monozygotic (MZ) twins Comparison of 5mC DNA Content and Histone H3 and H4 Acetylation Levels in MZ Twin Pairs.
H2B: 19 Lysines PEPAKSAPAPKKGSKKAVTKAQKKDGKERKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK 5 11 12 15 16 20 23 24 27 30 34 43 46 a1 57 85 L1 a2 L2 a3 108 116 120 125 LC aC
H2B: 14 modified Lysines (mass-spec) PEPAKSAPAPKKGSKKAVTKAQKKDGKERKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK Me Me Me Ac Ac Ac Ac Ac Ac Ac Ac 5 11 12 15 16 20 23 24 27 30 34 43 46 a1 Me Ac 57 85 L1 a2 L2 a3 Ub Ac Ac Ac 108 116 120 125 LC aC
H2B: abs against modified Lysines PEPAKSAPAPKKGSKKAVTKAQKKDGKERKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK Me Me Me Me Me Ac Ac Ac Ac Ac Ac Ac Ac Ac 5 11 12 15 16 20 23 24 27 30 34 43 46 a1 Me Ac 57 85 L1 a2 L2 a3 Ub Ac Ac Ac 108 116 120 125 LC aC
H2B: abs against K46ac, K46me2 PEPAKSAPAPKKGSKKAVTKAQKKDGKERKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK Me Me Me Me Ac Ac Ac Ac Ac Ac Ac Ac Ac 5 11 12 15 16 20 23 24 27 30 34 43 46 a1 ??? Me Ac 57 85 L1 a2 L2 a3 Ub Ac Ac Ac 108 116 120 125 LC aC
Histone methylation