Fig. 7. Dominant negative HNF-4 is able to rescue the estrogen inducibility of native FXII promoter by counteracting endogenous HNF-4. Native FXII promoter.

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Fig. 7. Dominant negative HNF-4 is able to rescue the estrogen inducibility of native FXII promoter by counteracting endogenous HNF-4. Native FXII promoter CAT reporter was cotransfected in HepG2 cells with expression vectors for ERα, HNF-4, and a DNA-binding domain-deleted HNF-4 (HNF-4DBD) or empty vector (−) using the same conditions as those described in Fig. 5. Data represent the average of four independent experiments, each performed in duplicate. Orphan Receptor Hepatocyte Nuclear Factor-4 Antagonizes Estrogen Receptor α-Mediated Induction of Human Coagulation Factor XII Gene**This work was supported by research grants from Ministero dell’Università e della Ricerca Scientifica e Tecnologica, Consiglio Nazionale delle Ricerche, Associazione Italiana per la Ricerca sul Cancro, Casa Sollievo Sofferenca Hospital, San Giovanni Rotondo, Italy. Endocrinology. 1998;139(11):4581-4589. doi:10.1210/endo.139.11.6299 Endocrinology | Copyright © 1998 by The Endocrine Society 1