SERVIZIO BIO-PLEX CRIBI Using this method, over 100 distinct microsphere sets can be created. It is important to remember that these beads, or microspheres are suspended in solution, so they are free to interact with the sample. This creates an assay platform that is - flexible (protein, peptides, DNA - reproducible ( as beads are uniformly distributed in solution) - improved sensitivity (better S/N, and near solution-phase kinetics) The object is to conjugate, or couple a different biological reaction to the surface of each bead set

Suspension Array reader Bio Plex A N L I S M U T E Suspension Array reader D I P R O T E N

Analisi qualitative e quantitative simultanee di proteine

Principio Funzionale Using this method, over 100 distinct microsphere sets can be created. It is important to remember that these beads, or microspheres are suspended in solution, so they are free to interact with the sample. This creates an assay platform that is - flexible (protein, peptides, DNA - reproducible ( as beads are uniformly distributed in solution) - improved sensitivity (better S/N, and near solution-phase kinetics) The object is to conjugate, or couple a different biological reaction to the surface of each bead set

Principio Funzionale Using this method, over 100 distinct microsphere sets can be created. It is important to remember that these beads, or microspheres are suspended in solution, so they are free to interact with the sample. This creates an assay platform that is - flexible (protein, peptides, DNA - reproducible ( as beads are uniformly distributed in solution) - improved sensitivity (better S/N, and near solution-phase kinetics) The object is to conjugate, or couple a different biological reaction to the surface of each bead set

Principio Funzionale Using this method, over 100 distinct microsphere sets can be created. It is important to remember that these beads, or microspheres are suspended in solution, so they are free to interact with the sample. This creates an assay platform that is - flexible (protein, peptides, DNA - reproducible ( as beads are uniformly distributed in solution) - improved sensitivity (better S/N, and near solution-phase kinetics) The object is to conjugate, or couple a different biological reaction to the surface of each bead set

La Camera di Lettura Using this method, over 100 distinct microsphere sets can be created. It is important to remember that these beads, or microspheres are suspended in solution, so they are free to interact with the sample. This creates an assay platform that is - flexible (protein, peptides, DNA - reproducible ( as beads are uniformly distributed in solution) - improved sensitivity (better S/N, and near solution-phase kinetics) The object is to conjugate, or couple a different biological reaction to the surface of each bead set

M U L T I W E B I O P L E X Reporter channel Classify channel To perform a test, thousands of probes, such as antibodies are bound to the microsphere. Reporter channel Classify channel

Saggi per Citochine

Sandwich Immunoassay - Cytokine - Saggi per Citochine Sandwich Immunoassay - Cytokine - IL-2 IL-4 TNFa GmCSF

Kit applicativi Citochine umane (27): Nuovo kit 27-plex: Multiplex tested Citochine umane (27): IL-2 IL-1b IL-4 IL-5 IL-6 IL-7 IL-8 IL-12 (p70) IL-10 IL-13 IFN- IL-17 TNF- MCP-1 (MCAF) GM-CSF MIP-1b Eotaxin Rantes IL-1ra IL-9 IL-15 PDGF bFGF VEGF Nuovo kit 27-plex: 171-A11127 HUMAN CYTOKINE 27-PLEX, 1X96

Kit applicativi Citochine di topo (23): Nuovo kit 23-plex: Multiplex tested Citochine di topo (23): IL-1 IL-1a MIP-1a IL-5 IL-3 Rantes IL-2 IL-6 IL-13 IL-10 IL-12 (p40) MCP1 IL-4 IL-12 (p70) Eotaxin IFN- IL-17 MIP-1 TNF- KC IL-9 GM-CSF Nuovo kit 23-plex: 171F11241 MOUSE CYTOKINE 23-PLEX, 1X96

Kit applicativi Citochine di Ratto (9): Kit 9-plex: Multiplex tested Citochine di Ratto (9): GM-CSF IL-4 IFN- IL-6 IL-1 IL-10 IL-2 TNF- Kit 9-plex: 171K11070 RAT CYTOKINES 9-PLEX A PANEL,1X96

Saggi per Fosfoproteine

Sandwich Immunoassay – Phospho - Saggi per Fosfoproteine Sandwich Immunoassay – Phospho - JNK IB ERK1 STAT3 P

Saggi per Fosfoproteine 15 Phosphoprotein Assays Phospho-Erk2 Phospho-IB- Phospho-JNK Phospho-p38 MAPK Phospho-Stat3 Phospho-ATF2 Phospho-Erk1 Phospho-Erk1/2 Phospho-EGFR Phospho-Akt Phospho-GSK-3a/b Phospho-p70 S6 Phospho-p90 RSK Phospho-Stat2 Phospho-TrkA Phospho C-Jun Phospho HSP 27 Phospho p53 Phospho NF-kβ Phospho S6 Ribosomal P. LISATI DI COLTURE CELLULARI LISATI DI CAMPIONI TISSUTALI

Saggi per Fosfoproteine 8 Total Target Assays Total Erk2 Total IB Total p38 MAPK Total ATF-2 Total JNK Total Akt Total p90 RSK Total c-Jun

Correlazione tra Bio-Plex e W. Blot All of our cytokine and phosphoprotein detection assays are validated against ELISA and western blot to validate the results. Here we look at the correlation between the Bio-Plex phosphoprotein detection assay and western blot for Erk and Stat-3. Phospho-Erk Phospho-Stat3 Western Blot, 8 ug protein/lane Western Blot, 8 ug protein/lane Bio-Plex Assay Bio-Plex Assay 1600 1200 MFI of Bio-Plex +2SD 800 400 1 2 3 4 5 6

Un Sistema Aperto Formati Applicabili Using this method, over 100 distinct microsphere sets can be created. It is important to remember that these beads, or microspheres are suspended in solution, so they are free to interact with the sample. This creates an assay platform that is - flexible (protein, peptides, DNA - reproducible ( as beads are uniformly distributed in solution) - improved sensitivity (better S/N, and near solution-phase kinetics) The object is to conjugate, or couple a different biological reaction to the surface of each bead set

Amine Coupling Kit

Amine Coupling Kit = o oH o + Chimica dell’accoppiamento C N H C N H R H C o N R H + = Carbodiimide reaction Le beads vengono attivate dall’EDC (1-ethyl-3 (3-dimethylaminopropyl) carbodiimide hydrocloride) Reagiscono con S-NHS (N-hydroxysuccinimide) Si formano esteri stabili che reagiscono con il gruppo NH2 della proteina per formare un legame covalente

Amine Coupling Kit 171-406001 171-5060XX Per 30 reazioni di accoppiamento 171-5060XX COOH Beads Regione XX, 1 ml Per 10 reazioni di accoppiamento Un coupling kit e 3 ml di beads sono sufficienti per 60 multiwell da 96 pozzetti

Saggi di Interazione DNA - Proteine

Interazione DNA-Proteine