Presentazione sul tema: "BioSInt Unit Superfici ed Interfacce biofunzionali Cecilia Pederzolli"— Transcript della presentazione:
1 BioSInt Unit Superfici ed Interfacce biofunzionali Cecilia Pederzolli Istituto "Marie Curie" PergineEleonora BusanaChiara CondlerStage estivi studenti
2 Laboratorio Scienze Biomoleculari ed Interfacce Centro Materiali e MicrosistemiCNR Centro Nazionale RicercheIBF Istituto di BiofisicaM. Dalla SerraStudio dei fenomeni che avvengono all’interfaccia - biosuperfici, biomembrane -con duplice obiettivo:Aumentare la comprensione dei meccanismi molecolari alla base delle malattieSviluppare sistemi innovativi di diagnosi e terapia (biomedicina)Settore di integrazione di diverse discipline: fisica, biologia, chimica
3 Surface properties play a crucial role BiointerfacesThe problemMaterials for medical devices have been selected on the basis of mechanical and physical properties, with less consideration given to their interactions with the biological environmentPoor interfacial biocompatibilityProtein-protein interactionCellCell-protein layer interactionProtein layerBIOMATERIALSurface properties play a crucial roleprotein adsorption, cell adhesion etc. are all surface-induce phenomena and depend on a large extent on the properties of the outer few atomic layers of materials
4 DNA surface immobilisation Surface modification processesto exercise a control over the way in which the biological fluids or individual biomolecules respond to the material surface1) Reduction of the aspecific adhesion2) Specific immobilisation of biomoleculessurface passivationligandblocking agentligandspacer armDNA surface immobilisationoligonucleotideGoal:Methods for covalent immobilization of biomolecules resulting in better biomolecule activity, reduced nonspecific adsorption, and greater stability.
5 Active multilayers deposited from liposomes Drug delivery systemsLipid-based nano-structured materialscell membranePartnershipCNR-IBF Trento, MIT BostonUsing micro nano-capsules (liposomes, particles) the transport and release of drugs and active molecules can be possible in a controlled waySystem- in solution- on implants surfaceActive principles- PFT bacterial toxins inhibitors- traditional antibacterial molecules- peptidesno treatmentRFMP treatment3x3 mInhibition of bacterial grownActive multilayers deposited from liposomes
6 Mix, Incubation & purification Sviluppo di materiali per la realizzazione di microsistemi per l’analisi del DNA in diagnostica molecolarebiological samplelysis bufferMix, Incubation & purificationEluted DNAtime scale (minutes)<120diagnosebloodMixerPCR mixPCR & detectioninherited diseasesInfections by pathogens...surface
7 Molecular diagnostics Surface analysis and chemical modification of silicon-based materials for the development of a lab-on-chip capable to perform a genetic analysis starting from the extraction of genomic DNA directly from blood to the genetic identification of specific mutations and/or SNPs (single nucleotide polymorphism).DNA INDNA OUT1st channel10th channelChannels 2-9PicoGreen stained genomic DNAThe system is a silicon/pyrex microchip (1x1cm, 6 µL total volume), where whole blood is injected (0.5 µL), DNA is extracted and used as a template for on-chip PCR. Products are revealed by fluorescence detection directly on chip.PartnershipOlivetti Jet, Biodiversity, Telethon, Politecnico di Torino
8 Molecular diagnostics Research overview Carbon nanotube-based sensors for DNA detectionSEM and TEM analysisCNT – fluorescein π stacking interactionECL detection by means of Ru(bpy)32+ labelled DNA1. Realization of electric contact on CNT cylinder2. CNT embedding in PDMS matrix3. Thermal treatment at 100 °C4. Electrode detaching from silicon support5. Electric conductivity testElectrodesAs expected, the maximum ECL intensity falls in correspondence of Ru(bpy)32+ electrochemical oxidation (+1.2 V vs. Ag)The first of three voltammetric cycles is indicated by solid line, while the subsequent two by dashed lines.The curve modifications from first to third cycle are indicated by the arrow directionsPartnershipPolitecnico di Torino, University of Bologna
9 Il gruppo di ricerca Valeria Antonini Ramona Dallapiccola Lorenzo LunelliMarta MarchiorettoLorenza MarocchiLaura PasquardiniFederica PirasCristina PotrichMayra TejucaLaura TosattoMichele VinanteManuela Zanetti
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