Laboratorio Scienze Biomoleculari ed Interfacce Centro Materiali e Microsistemi CNR Centro Nazionale Ricerche IBF Istituto di Biofisica M. Dalla Serra Studio dei fenomeni che avvengono allinterfaccia - biosuperfici, biomembrane - con duplice obiettivo: Aumentare la comprensione dei meccanismi molecolari alla base delle malattie Sviluppare sistemi innovativi di diagnosi e terapia (biomedicina) Settore di integrazione di diverse discipline: fisica, biologia, chimica
Protein-protein interaction Cell-protein layer interaction BIOMATERIAL Protein layer Biointerfaces The problem Materials for medical devices have been selected on the basis of mechanical and physical properties, with less consideration given to their interactions with the biological environment Poor interfacial biocompatibility Surface properties play a crucial role protein adsorption, cell adhesion etc. are all surface-induce phenomena and depend on a large extent on the properties of the outer few atomic layers of materials Cell
to exercise a control over the way in which the biological fluids or individual biomolecules respond to the material surface surface passivation ligand blocking agent + + + + ligand spacer arm DNA surface immobilisation oligonucleotide 1) Reduction of the aspecific adhesion 2) Specific immobilisation of biomolecules Goal: Methods for covalent immobilization of biomolecules resulting in better biomolecule activity, reduced nonspecific adsorption, and greater stability. Surface modification processes
Drug delivery systems Using micro nano-capsules (liposomes, particles) the transport and release of drugs and active molecules can be possible in a controlled way Inhibition of bacterial grown 3x3 m System - in solution - on implants surface Active principles - PFT bacterial toxins inhibitors - traditional antibacterial molecules - peptides Active multilayers deposited from liposomes cell membrane Lipid-based nano-structured materials CNR-IBF Trento, MIT Boston Partnership no treatment RFMP treatment
biological sample lysis buffer Mix, Incubation & purification Eluted DNA time scale (minutes) 0 <120 diagnose blood Mixer PCR mix PCR & detection Sviluppo di materiali per la realizzazione di microsistemi per lanalisi del DNA in diagnostica molecolare surface inherited diseases Infections by pathogens...
Molecular diagnostics Surface analysis and chemical modification of silicon-based materials for the development of a lab-on-chip capable to perform a genetic analysis starting from the extraction of genomic DNA directly from blood to the genetic identification of specific mutations and/or SNPs (single nucleotide polymorphism). The system is a silicon/pyrex microchip ( 1x1cm, 6 µL total volume), where whole blood is injected (0.5 µL), DNA is extracted and used as a template for on-chip PCR. Products are revealed by fluorescence detection directly on chip. Olivetti Jet, Biodiversity, Telethon, Politecnico di Torino Partnership DNA IN DNA OUT 1 st channel 10 th channel Channels 2-9 PicoGreen stained genomic DNA
Research overview Carbon nanotube-based sensors for DNA detection CNT – fluorescein π stacking interaction ECL detection by means of Ru(bpy) 3 2+ labelled DNA As expected, the maximum ECL intensity falls in correspondence of Ru(bpy) 3 2+ electrochemical oxidation (+1.2 V vs. Ag) Molecular diagnostics SEM and TEM analysis 1. Realization of electric contact on CNT cylinder 2. CNT embedding in PDMS matrix 3. Thermal treatment at 100 °C 4. Electrode detaching from silicon support 5. Electric conductivity test Electrodes Politecnico di Torino, University of Bologna Partnership The first of three voltammetric cycles is indicated by solid line, while the subsequent two by dashed lines. The curve modifications from first to third cycle are indicated by the arrow directions
Valeria Antonini Ramona Dallapiccola Lorenzo Lunelli Marta Marchioretto Lorenza Marocchi Laura Pasquardini Federica Piras Cristina Potrich Mayra Tejuca Laura Tosatto Michele Vinante Manuela Zanetti Il gruppo di ricerca