hnRNP Circa 30 proteine abbondanti identificate su gel bidimensionale Contengono motivi di legame all’RNA (RRM) e altri domini “ausiliari” I complessi hnRNP contengono circa 700 nt di RNA Molte hnRNP fanno da “navetta” tra nucleo a citoplasma
Splicing nucleare Splicing di introni di tipo II Splicing di introni di tipo I Splicing di tRNA di lievito spliceosoma nucleasi e ligasi autosplicing trans- esterificazioni meccanismo simile Tipi di splicing
Figure 2 Potential base pairing interactions between the branch site of the human P120 AT–AC intron and U12 or U2 snRNAs. The adenosine residue that forms the lariat structure is marked by an asterisk and shown bulged from the putative intron–snRNA hybrid. Possible base pairings are indicated by vertical lines. The sites in the P120 intron where mutations inhibit splicing in vivo are marked in bold, as are the corresponding two sites in U12 snRNA where compensatory mutations that restore the potential for base pairing suppress the splicing defect
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